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superscript® iii reverse transcriptase synthesis kit  (Thermo Fisher)


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    Thermo Fisher superscript® iii reverse transcriptase synthesis kit
    Superscript® Iii Reverse Transcriptase Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iii+reverse+transcriptase/superscript++iii+reverse+transcriptase+synthesis+kit/pmc12221670-72-18-24
    Average 90 stars, based on 1 article reviews
    superscript® iii reverse transcriptase synthesis kit - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: TERRA R-loops trigger a switch in telomere maintenance towards break-induced replication and PRIMPOL-dependent repair.
    Article Snippet: .. For RT-qPCR analysis of TERRA levels, 3 μg of RNA was reverse transcribed using 200 U of SuperScript III reverse transcriptase (ThermoFisher Scientific), along with GAPDH and TERRA reverse primers (see “Reagents and Tools Table”). ..

    Reverse Transcription:

    Article Title: TERRA R-loops trigger a switch in telomere maintenance towards break-induced replication and PRIMPOL-dependent repair.
    Article Snippet: .. For RT-qPCR analysis of TERRA levels, 3 μg of RNA was reverse transcribed using 200 U of SuperScript III reverse transcriptase (ThermoFisher Scientific), along with GAPDH and TERRA reverse primers (see “Reagents and Tools Table”). ..

    Article Title: Deletion viral genome diversity among bovine viral diarrhea virus (BVDV) 1a and 1b strains.
    Article Snippet: .. At the time of plaque picking after each passage at 2 different MOIs, viral RNA was extracted, cDNA was generated via Superscript III reverse transcriptase (Thermo Fisher Scientific, Inc.), and whole-genome sequencing was conducted as previously described and detailed above [45, 52]. .. Statistical analysis tests were performed as indicated in each figure legend via GraphPad Prism version 10.2.0 (GraphPad Software, Inc.).

    Article Title: p66Shc deletion confers apoptotic resistance to loss of EGFR-ERK signalling in neural stem cells.
    Article Snippet: Genomic DNA was removed using the RNaseFree DNase Set (Qiagen). .. First-strand cDNA synthesis was performed using SuperScript III Reverse Transcriptase (Invitrogen, ThermoFisher). .. Quantitative PCR was carried out using the SensiFASTTM SYBR No-ROX Kit (FroggaBio) on a CFX384 real-time thermal cycler (Bio-Rad).

    Article Title: Epitranscriptomic Modulation of TET2 Inhibition Suppressed SARS-CoV-2 Infection and Blocked Viral Nucleocapsid Protein in Induced-Pluripotent-Stem-Cell-Derived Cardiomyocyte Screening Models
    Article Snippet: .. Following previous protocols [ ], complementary DNA synthesis was carried out using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific) with 500 to 1,000 ng of total RNA as input. ..

    Article Title: Single-cell RNA sequencing clarifies dermal fibroblast subset representation in vitro and reveals variable persistence of keloid disease-associated features
    Article Snippet: .. RNA was reverse transcribed using SuperScript III reverse transcriptase (200U/μL) with dithiothreitol (0.1M) and first-strand buffer (5X) (Invitrogen, #18080093) and random primers (Invitrogen, #48190011) plus dNTPs (Invitrogen, #18427013) according to manufacturer’s instructions. .. Due to low RNA yield (<4ng/μL) from some samples, RNA from all samples for the P0-P4 sequencing validation experiment were amplified and converted to cDNA using the Smart-seq2 protocol (steps 1 to 27) ( ). cDNA was diluted to 2ng/μL, and qPCR was performed using a Roche LightCycler 480II with SYBR Green I (2x; Roche, #04887352001) according to manufacturer’s instructions.

    Article Title: Histone lysine methyltransferases MLL3 and MLL4 direct gene expression to produce platelets efficiently.
    Article Snippet: .. RNAs were reverse transcribed into cDNA using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific). qPCR was then performedwith iTaqUniversal SYBR® Green Supermix (Bio-Rad) on anABI 7500 Fast Real-Time PCR system, using the primers described in Supplementary Data 1. ..

    Article Title: Sensing of extracellular l-proline availability by the integrated stress response determines the outcome of cell competition.
    Article Snippet: .. RNA extraction and quantitative reverse transcription polymerase chain reaction RNA was extracted with the RNeasy mini kit (QIAGEN), and SuperScript III reverse transcriptase (Thermo Fisher Scientific) was used for cDNA synthesis according to the manufacturer’s instructions. .. Quantitative reverse transcription polymerase chain reaction (RT- PCR) was performed by amplification with LightCycler 480 SYBR Green Master (Roche).

    Generated:

    Article Title: Deletion viral genome diversity among bovine viral diarrhea virus (BVDV) 1a and 1b strains.
    Article Snippet: .. At the time of plaque picking after each passage at 2 different MOIs, viral RNA was extracted, cDNA was generated via Superscript III reverse transcriptase (Thermo Fisher Scientific, Inc.), and whole-genome sequencing was conducted as previously described and detailed above [45, 52]. .. Statistical analysis tests were performed as indicated in each figure legend via GraphPad Prism version 10.2.0 (GraphPad Software, Inc.).

    Article Title: Deletion viral genome diversity among bovine viral diarrhea virus (BVDV) 1a and 1b strains
    Article Snippet: .. At the time of plaque picking after each passage at 2 different MOIs, viral RNA was extracted, cDNA was generated via Superscript III reverse transcriptase (Thermo Fisher Scientific, Inc.), and whole-genome sequencing was conducted as previously described and detailed above [ , ]. .. Statistical analysis tests were performed as indicated in each figure legend via GraphPad Prism version 10.2.0 (GraphPad Software, Inc.).

    Sequencing:

    Article Title: Deletion viral genome diversity among bovine viral diarrhea virus (BVDV) 1a and 1b strains.
    Article Snippet: .. At the time of plaque picking after each passage at 2 different MOIs, viral RNA was extracted, cDNA was generated via Superscript III reverse transcriptase (Thermo Fisher Scientific, Inc.), and whole-genome sequencing was conducted as previously described and detailed above [45, 52]. .. Statistical analysis tests were performed as indicated in each figure legend via GraphPad Prism version 10.2.0 (GraphPad Software, Inc.).

    Article Title: Deletion viral genome diversity among bovine viral diarrhea virus (BVDV) 1a and 1b strains
    Article Snippet: .. At the time of plaque picking after each passage at 2 different MOIs, viral RNA was extracted, cDNA was generated via Superscript III reverse transcriptase (Thermo Fisher Scientific, Inc.), and whole-genome sequencing was conducted as previously described and detailed above [ , ]. .. Statistical analysis tests were performed as indicated in each figure legend via GraphPad Prism version 10.2.0 (GraphPad Software, Inc.).

    cDNA Synthesis:

    Article Title: p66Shc deletion confers apoptotic resistance to loss of EGFR-ERK signalling in neural stem cells.
    Article Snippet: Genomic DNA was removed using the RNaseFree DNase Set (Qiagen). .. First-strand cDNA synthesis was performed using SuperScript III Reverse Transcriptase (Invitrogen, ThermoFisher). .. Quantitative PCR was carried out using the SensiFASTTM SYBR No-ROX Kit (FroggaBio) on a CFX384 real-time thermal cycler (Bio-Rad).

    Article Title: Sensing of extracellular l-proline availability by the integrated stress response determines the outcome of cell competition.
    Article Snippet: .. RNA extraction and quantitative reverse transcription polymerase chain reaction RNA was extracted with the RNeasy mini kit (QIAGEN), and SuperScript III reverse transcriptase (Thermo Fisher Scientific) was used for cDNA synthesis according to the manufacturer’s instructions. .. Quantitative reverse transcription polymerase chain reaction (RT- PCR) was performed by amplification with LightCycler 480 SYBR Green Master (Roche).

    DNA Synthesis:

    Article Title: Epitranscriptomic Modulation of TET2 Inhibition Suppressed SARS-CoV-2 Infection and Blocked Viral Nucleocapsid Protein in Induced-Pluripotent-Stem-Cell-Derived Cardiomyocyte Screening Models
    Article Snippet: .. Following previous protocols [ ], complementary DNA synthesis was carried out using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific) with 500 to 1,000 ng of total RNA as input. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Histone lysine methyltransferases MLL3 and MLL4 direct gene expression to produce platelets efficiently.
    Article Snippet: .. RNAs were reverse transcribed into cDNA using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific). qPCR was then performedwith iTaqUniversal SYBR® Green Supermix (Bio-Rad) on anABI 7500 Fast Real-Time PCR system, using the primers described in Supplementary Data 1. ..

    SYBR Green Assay:

    Article Title: Histone lysine methyltransferases MLL3 and MLL4 direct gene expression to produce platelets efficiently.
    Article Snippet: .. RNAs were reverse transcribed into cDNA using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific). qPCR was then performedwith iTaqUniversal SYBR® Green Supermix (Bio-Rad) on anABI 7500 Fast Real-Time PCR system, using the primers described in Supplementary Data 1. ..

    RNA Extraction:

    Article Title: Sensing of extracellular l-proline availability by the integrated stress response determines the outcome of cell competition.
    Article Snippet: .. RNA extraction and quantitative reverse transcription polymerase chain reaction RNA was extracted with the RNeasy mini kit (QIAGEN), and SuperScript III reverse transcriptase (Thermo Fisher Scientific) was used for cDNA synthesis according to the manufacturer’s instructions. .. Quantitative reverse transcription polymerase chain reaction (RT- PCR) was performed by amplification with LightCycler 480 SYBR Green Master (Roche).

    Polymerase Chain Reaction:

    Article Title: Sensing of extracellular l-proline availability by the integrated stress response determines the outcome of cell competition.
    Article Snippet: .. RNA extraction and quantitative reverse transcription polymerase chain reaction RNA was extracted with the RNeasy mini kit (QIAGEN), and SuperScript III reverse transcriptase (Thermo Fisher Scientific) was used for cDNA synthesis according to the manufacturer’s instructions. .. Quantitative reverse transcription polymerase chain reaction (RT- PCR) was performed by amplification with LightCycler 480 SYBR Green Master (Roche).



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